Transcriptional activation of RACTK1 K+ channel gene by apical alkalization in renal cortical collecting duct cells.
نویسندگان
چکیده
منابع مشابه
Transcriptional activation of RACTK1 K+ channel gene by apical alkalization in renal cortical collecting duct cells.
We have previously demonstrated that RACTK1 cDNA encodes a pH sensitive K+ channel expressed in the apical side of renal collecting tubule cells. To determine whether extracellular pH induces the RACTK1 gene expression in the renal cortical collecting duct (CCD) cells, we measured mRNA of the RACTK1 using cultured rabbit CCD cells. Alkalization of incubation medium activated the transcription o...
متن کاملMineralocorticoids decrease the activity of the apical small-conductance K channel in the cortical collecting duct.
We used the patch-clamp technique to examine the effect of DOCA treatment (2 mg/kg) on the apical small-conductance K (SK) channels, epithelial Na channels (ENaC), and the basolateral 18-pS K channels in the cortical collecting duct (CCD). Treatment of rats with DOCA for 6 days significantly decreased the plasma K from 3.8 to 3.1 meq and reduced the activity of the SK channel, defined as NP(o),...
متن کاملRenal Collecting Duct Cells
1 2 August 3, 2012 3 Revised: August 6, 2012 4 5 Quantitative Phosphoproteomics in Nuclei of Vasopressin-Sensitive 6 Renal Collecting Duct Cells 7 8 9 Steven J. Bolger*, Patricia A. Gonzales Hurtado*, Jason D. Hoffert, Fahad Saeed, Trairak 10 Pisitkun, and Mark A. Knepper 11 12 *Co-First Authors 13 14 Epithelial Systems Biology Laboratory, 15 NHLBI, National Institutes of Health, 16 Bethesda, M...
متن کاملTranscriptional control of renal collecting duct development.
METANEPHRIC KIDNEY DEVELOPMENT is a complex process involving maturational changes and reciprocal signaling between epithelial and mesenchymal tissues. Over the last 15–20 years, the number of reports on kidney development has increased, with the vast majority focused on early events such as interactions between the ureteric bud and metanephric mesenchyme, ureteric bud branching morphogenesis, ...
متن کاملMechanism of activation of ERK and H-K-ATPase by isoproterenol in rat cortical collecting duct.
Isoproterenol stimulates H-K-ATPase activity in rat cortical collecting duct beta-intercalated cells through a PKA-dependent pathway. This study aimed at determining the signaling pathway underlying this effect. H-K-ATPase activity was determined in microdissected collecting ducts preincubated with or without specific inhibitors or antibodies against intracellular signaling proteins. Transient ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1996
ISSN: 0021-9738
DOI: 10.1172/jci118814